Co-crystallization Xaperones

We deliver panels of high affinity in vivo matured Xaperones¹ that facilitate the crystallization of your proteins of interest in a competitive time frame:

• We identify Xaperones against your epitope of interest.
• We identify Xaperones that selectively stabilize unique therapeutic conformations of your targets.

Standard deals require native, purified protein provided by the customer and include:

• Up to 20 different Xaperones for a given target.
• Xaperone affinities in the range of 0.1 to 100 nM.
• Recombinant Xaperones produced as His-tagged protein in the periplasm of E. coli (1 to 10 mg/liter). 
• The Xaperone sequences, the materials (cells and plasmids) and the protocols to produce and purify them in E. coli. 
  

In the absence of purified protein, we generate Xaperones against membrane proteins. 

Xaperone aided structure determination

For structure determination, each project is subdivided in phases that may be performed individually at customer’s request:

• Production of Xaperones.
• Co-crystallization trials.
• Co-crystal optimisation.
• Data collection and structure determination (X-ray or NMR).

¹ Note that Xaperones can only be used for protein crystallization purposes. In case the customer has the intention to use Xaperones for other purposes, such as therapeutic or diagnostic use, contact us immediately.